Adipose derived mesenchymal stem cells improve diabetic wound healing in mouse animal model: extracellular matrix remodeling maybe a potential therapeutic usage of stem cells

Skin wounds cause damage to the body's first layer of protection. This, disclosed a person to further injury. Wounds normally heal in a very orderly and efficient process. However, activation of this efficient process is sometimes lost in pathologic conditions such as diabetes. The objective of the present study was to evaluate the expression of some genes in Adipose Derived Mesenchymal Stem Cells (ADSCs) that were used for the healing of the diabetic wound of mouse. ADSCs were separated from adipose tissue of mice, confirmed by surface markers CD34, CD105, CD44 and bone or fat cells differentiation. Then 10 × 105 stem cells were immediately injected in four areas around the wound that previously were created on the dorsal skin of each diabetic mouse. TIMP-1, MMP-2, MMP-9 and uPA genes expression folds in the wound area were examined on 3rd, 7th, 14th and 21st days, using q-PCR. Three groups’ mice were evaluated: non-diabetic, diabetic without any treatment and diabetic with ADSCs treatment. The expression level of uPA and MMP-9 genes were decreased in the stem cell-treated group but TIMP1 and MMP2 genes folds were increased significantly (p<0.05) compared to the diabetes group without any treatment. The results of this study suggest that stem cell transplantation maybe applicable in diabetic wounds healing via changing the content of tissue gene expressions.